Vermitechnology transforms hazardous red mud into benign organic input for agriculture: Insights on earthworm-microbe interaction, metal removal, and soil-crop improvement.

Bioremediation of hazardous bauxite residues, red mud (RM), through vermicomposting has yet to be attempted. Therefore, the valorization potential of Eisenia fetida in various RM and cow dung (CD) mixtures was compared to aerobic composting. Earthworm fecundity and biomass growth were hindered in RM + CD (1:1) feedstock but enhanced in RM + CD (1:3). The pH of highly alkaline RM-feedstocks sharply reduced (>17%) due to vermicomposting. N, P, and K availability increased dramatically with Ca and Na reduction under vermicomposting. Additionally, ∼40-60% bioavailable metal fractions were transformed to obstinate (organic matter and residual bound) forms upon vermicomposting. Consequently, the total metal concentrations were significantly reduced with considerably high earthworm bioaccumulation. Microbial growth and enzyme activity were more significant under vermicomposting than composting. Correlation statistics revealed that microbial augmentation significantly facilitated a metal reduction in RM-vermibeds. Eventually, RM-vermicompost stimulated sesame growth and improved soil health with the least heavy metal contamination to soil and crop.

Polycyclic aromatic hydrocarbon (PAH) remediation during vermicomposting and composting: Mechanistic insights through PAH-budgeting.

Knowledge on the mechanism of earthworm-induced removal of polycyclic aromatic hydrocarbons (PAH) in vermicomposting systems and interaction with nutrient mineralization and microbial growth is scarce in literature. Moreover, the PAH accumulation capacity of Eudrilus eugeniae has not been studied. This research, therefore, investigates the apportionment dynamics of 13 PAH compounds in aerobic composting and vermicomposting (Eisenia fetida and E. eugeniae) systems using novel budget equations. The PAH removal efficiency of vermicomposting was significantly higher (2-threefold) than composting with concurrent microbial augmentation (p < 0.01). However, the 4-6 ring compounds reduced more significantly (30-50%) than the 3-ring PAHs (p < 0.01), and E. eugeniae was an equally competitive PAH-accumulator compared to E. fetida. The budget equations revealed that although the bioaccumulation capabilities of earthworms were retarded due to PAH exposure, earthworms facilitated PAH-immobilization in decomposed feedstock. A marked increase in bacterial, fungal, and actinomycetes proliferation in PAH-spiked vermibeds with parallel removal of the PAHs indicated that earthworm-induced microbial enrichment plays a vital role in PAH detoxification during vermicomposting. Correlation analyses strongly implied that earthworm-driven mineralization-humification balancing and microbial enrichment could be the critical mechanism of PAH remediation under vermicomposting.

Exorbitant signatures of pesticides and pharmaceuticals in municipal solid wastes (MSWs): Novel insights through risk analysis, dissolution dynamics, and model-based source identification.

Studies on the occurrence and fates of emerging organic micropollutants (EOMPs) like pharmaceuticals and pesticides in MSWs are scarce in the literature. Therefore, MSWs were sampled from 20 Indian landfills and characterized for five widely consumed EOMPs (chlorpyrifos, cypermethrin, carbofuran, carbamazepine, and sodium diclofenac), physicochemical, and biological properties. The pesticide (median: 0.17-0.44 mg kg-1) and pharmaceutical (median: 0.20-0.26 mg kg-1) concentrations significantly fluctuated based on landfill localities. Eventually, principal component and multi-factor (MFA) models demonstrated close interactions of EOMPs with biological (microbial biomass and humification rates) and chemical (N, P, K, Ca, S, etc.) properties of MSWs. At the same time, the MFA resolved that EOMPs' fates in MSWs significantly differ from bigger cosmopolitan cities to smaller rural townships. Correspondingly, the concentration-driven ecological risks were high in 15 MSWs with EOMP-toxicity ranks of diclofenac > carbofuran = chlorpyrifos > cypermethrin > carbamazepine. The EOMPs' dissolution dynamics and source apportionments were evaluated using the positive matrix factorization (PMF) model for the first time on experimental data, extracting four anthropogenic sources (households, heterogeneous business centers, agricultural, and open drains). The most significant contribution of EOMPs to MSWs was due to heterogeneous business activity. Notably, the aging of soluble chemical fractions seems to influence the source characteristics of EOMPs strongly.

Capsaicin derived from endemic chili landraces combats Shigella pathogen: Insights on intracellular inhibition mechanism.

Ethnic tribals in northeast India have been growing and maintaining local chili landraces for ages. These chilies are known for their characteristic pungency and immense therapeutic properties. Capsaicin, a significant chili metabolite, is recognized as a natural drug for pain relief, diabetic neuropathy, psoriasis, arthritis, etc. In this study, we tried to observe the influence of locality factors on the pungency and bioactive features of Capsicum annuum L. landraces. We also checked the gastro-protective ability of these chilies, especially in the cure of shigellosis. Phytometabolite characterization and estimation were done through spectrophotometric methods. Preparative and analytical HPLC techniques were employed for extracting and purifying capsaicin-enriched fractions. Shigella flexneri growth retardation was determined through the broth dilution method. Gentamicin protection assay and ELISA were done to assess the intracellular invasion and IL-1ß inflammasome production by S.flexneri. The correlation analyses postulated that phenols, flavonoids, chlorophylls, ß-carotene, and capsaicin synthase upregulation strongly influenced capsaicin biosynthesis in chili cultivars. Correspondingly, the inhibitory efficacy of the HPLC-purified Balijuri-derived capsaicin was more effective than the Raja-derived capsaicin in inhibiting intracellular Shigella growth. Reduced levels of pro-inflammatory cytokine (IL1ß) in capsaicin-treated Shigella-infected cells probably reduced inflammation-mediated intestinal damage, limiting bacterial spread. This investigation advocates the unique potential of local chilies in curing deadly 'shigellosis' with mechanistic evidence. Our observation justifies the traditional healing practices of the ethnic people of NE India.

Earthworm stocking density regulates microbial community structure and fatty acid profiles during vermicomposting of lignocellulosic waste: Unraveling the microbe-metal and mineralization-humification interactions.

Earthworm-induced microbial enrichment is the key to success in vermitechnology, yet the influence of initial earthworm stocking density on microbial community profiles in vermibeds is unknown. Therefore, vermicomposting of lignocellulosic feedstock was performed with different stocking densities of two earthworms (Eisenia fetida and Eudrilus eugeniae) compared with composting. Eventually, earthworm growth, microbial (activity and community profiles), and physicochemical dynamics were assessed. The earthworm population significantly increased under low stocking, while denser stocking (15/kg) was stressful. The XRD-based crystallinity assessment revealed that comminuting efficiency of Eisenia and Eudrilus was prudent at 7 and 10 worm/kg stockings, respectively. Moreover, the 5 and 7 worm/kg stockings effectively mobilized microbial activity, promoting NPK-mineralization and C-humification balance. Correlation statistics indicated that earthworm stocking density-driven microbial community shift and fatty acid profiles strongly influenced metal removal in vermibeds. Hence, the findings implied that 5-7 worm/kg stockings of earthworms produced high-quality sanitized vermicompost.

Eco-geological consequences of textile processing wastes: Risk assessment, elemental dissolution kinetics, and health hazard potential.

Although substantial quantities of toxic wastes are generated from textile industries, the characteristics of textile processing wastes (TPWs) have yet scantily been investigated from ecological and agricultural perspectives. Here, the eco-geological consequences of TPWs are evaluated by considering three types of sludges (i.e., silk fibre sludge (SFS), dye mixed silk processing sludge (DSPS), and cotton processing wastewater sludge (CPWS)). The predominance of certain components between different wastes (e.g., fibrous substances in silk industry wastes (i.e., SFS and DSPS) and amorphous materials in cotton processing wastes (i.e., CPWS)) is accounted for by the use of different raw materials in different industries. According to the FTIR and other characterization analyses, all three types of TPWs were rich in carbonaceous compounds and nutrients (e.g., CNPK) because of their biological origin. Further, high accumulation of toxic metals (e.g., Cd, Cr, Cu, Zn, Pb, and Mn) was apparent with chemical-processing routes. The principal component analysis indicated strong relationships between certain environmental variables (e.g., moisture content and bulk density) and bioavailability of several metals (e.g., Cd, Zn, Cu, and Mn), while C levels in TPWs were tightly associated with Cr levels. According to the Visual MINTEQ model, the dissolution-precipitation dynamics of potentially toxic elements (e.g., Pb, Cr, and Zn) in TPWs are predicted to be controlled by the levels of phosphates/chlorides/sulphates in line with the textile processing steps employed in different factories. The great toxicity potential of CPWS (e.g., relative to SFS and DSPS) is recognized to pose significant metal-induced hazards to ecosystems and human health over time. Among the three TPWs, SFS could be prescribed for agricultural application after proper treatment (e.g., via valorization techniques) with the aid of its benign nature and high nutrient (Total N: 3.83%; available P: 118.6 mg kg-1) value.

Capsaicin Inhibits Shigella flexneri Intracellular Growth by Inducing Autophagy.

Antibiotic treatment plays an essential role in preventing Shigella infection. However, incidences of global rise in antibiotic resistance create a major challenge to treat bacterial infection. In this context, there is an urgent need for newer approaches to reduce S. flexneri burden. This study largely focuses on the role of the herbal compound capsaicin (Caps) in inhibiting S. flexneri growth and evaluating the molecular mechanism behind bacterial clearance. Here, we show for the first time that Caps inhibits intracellular S. flexneri growth by inducing autophagy. Activation of autophagy by Caps is mediated through transcription factor TFEB, a master regulator of autophagosome biogenesis. Caps induced the nuclear localization of TFEB. Activation of TFEB further induces the gene transcription of autophagosomal genes. Our findings revealed that the inhibition of autophagy by silencing TFEB and Atg5 induces bacterial growth. Hence, Caps-induced autophagy is one of the key factors responsible for bacterial clearance. Moreover, Caps restricted the intracellular proliferation of S. flexneri-resistant strain. The efficacy of Caps in reducing S. flexneri growth was confirmed by an animal model. This study showed for the first time that S. flexneri infection can be inhibited by inducing autophagy. Overall observations suggest that Caps activates TFEB to induce autophagy and thereby combat S. flexneri infection.

Ward based goal directed fluid therapy (GDFT) in acute pancreatitis (GAP) trial: A feasibility randomised controlled trial.

BACKGROUND: Goal-directed fluid therapy (GDFT) reduces complications in patients undergoing major general surgery. There are no reports of cardiac output evaluation being used to optimise the fluid administration for patients with acute pancreatitis (AP) in a general surgery ward. METHOD: 50 patients with AP were randomised to either ward-based GDFT (n = 25) with intravenous (IV) fluids administered based on stroke volume optimisation protocol or standard care (SC) (n = 25), but with blinded cardiac output evaluation, for 48-h following hospital admission. Primary outcome was feasibility. RESULTS: 50 of 116 eligible patients (43.1%) were recruited over 20 months demonstrating feasibility. 36 (72%) completed the 48-h of GDFT; 10 (20%) discharged within 48-h and 4 withdrawals (3 GDFT, 1 SC). Baseline characteristics were similar with only 3 participants having severe disease (6%, 1 GDFT, 2 SC). Similar volumes of IV fluids were administered in both groups (GDFT 5465 (1839) ml, SC 5211 (1745) ml). GDFT group had a lower heart rate, blood pressure and respiratory rate and improved oxygen saturations. GDFT was not associated with any harms. There was no evidence of difference in complications of AP (GDFT 24%, SC 32%) or in the duration of stay in intensive care (GDFT 0 (0), SC 0.7 (3) days). Length of hospital stay was 5 (2.9) days in GDFT and 6.3 (7.6) in SC groups. CONCLUSION: Ward-based GDFT is feasible and shows a signal of possible efficacy in AP in this early-stage study. A larger multi-site RCT is required to confirm clinical and cost effectiveness.

Simultaneous early surgical repair of post-cholecystectomy major bile duct injury and complex abdominal evisceration: A case report.

BACKGROUND: Major bile duct injuries (BDIs) are hazardous complications during 0.4%-0.6% of laparoscopic cholecystectomies. Major BDIs usually require surgical repair, ideally either immediately or at least six weeks after the damage. The complexity of our case lies in the coexistence of early BDI followed by 2-week biliary peritonitis with massive midline evisceration which, in combination, has over 40% mortality risk. METHODS & CASE REPORT: We describe the case of a 65-year-old male, transferred to our tertiary HPB service on day 14 after common bile duct complete transection during cholecystectomy and postoperative laparotomy. The patient presented with biliary peritonitis along with full wound dehiscence and extensive evisceration. During emergency peritoneal wash-out surgery we deemed immediate BDI repair feasible by primary Roux-en-Y hepaticojejunostomy (HJ), with multi-stage abdominal closure. In the following days we performed progressive abdominal wall closure in multiple sessions under general anesthesia, aided by vacuum-assisted wound closure and intraperitoneal mesh-mediated fascial traction-approximation (VAWCM) with permeable mesh. An expected late incisional hernia was eventually repaired through component separation and biological mesh. DISCUSSION & CONCLUSION: The simultaneous use of Roux-en-Y HJ and VAWCM has proven safe and effective in the treatment of BDI and 2-week biliary peritonitis with massive midline evisceration.

Amine-amide functionalized graphene oxide sheets as bifunctional adsorbent for the removal of polar organic pollutants.

Effective mitigation of polar organic impurities from industrial effluents is a global environmental challenge. Here, we describe the solvothermal synthesis of ammonia-functionalized graphene oxide (NH3GO) sheets for adsorptive removal of diverse organic pollutants, such as cationic dye basic blue 41 (BB41), anionic dye methyl orange (MO), and ionic 4-nitrophenol (4-NP), in aqueous media. Structural analysis of NH3GO suggest a potent role of surface acidic and basic binding sites in adsorption of targets through an interplay of dynamic experimental variables, e.g., contact time, pH, initial adsorbate concentration, adsorbent mass, and temperature. At an initial pollutant concentration of 20 mg/L, equilibrium adsorption capacities for BB41, MO, and 4-NP were estimated at 199.5, 64.0, and 54.1 mg/g, respectively, with corresponding partition coefficients of 4156, 79.4, and 14.3 L/g, respectively. Experimental data of all three organic pollutants are best fitted by the pseudo-second-order kinetic model. The adsorption isotherm of BB41 follows a multilayer adsorption pattern, while those of MO and 4-NP fit into a monolayer adsorption pattern. The endothermic and spontaneous nature of the adsorption processes has also been explored for the three targets on NH3GO based on thermodynamic analysis. The prepared NH3GO sheets appear to be a promising adsorbent for the removal of polar organic dyes and aromatics in the solution phase.

Assessing the arsenic-saturated biochar recycling potential of vermitechnology: Insights on nutrient recovery, metal benignity, and microbial activity.

Biochar mediated pollutant removal is gaining attention because of high efficiency of the process. However, effective recycling avenues of the pollutant-saturated biochars are scarce in the knowledge base; while such materials can be a new source of long-range contamination. Therefore, potential of vermitechnology for eco-friendly recycling of pollutant-loaded biochar was assessed by using arsenic-saturated native (NBC) and exfoliated (EBC) biochars as feedstocks for the first time. Interestingly, the bioavailable arsenic fractions (water soluble and exchangeable) considerably reduced by 22-44 % with concurrent increment (~8-15 %) of the recalcitrant (residual and organic bound) fractions in the biochar-based feedstocks. Consequently, ~2-3 folds removal of the total arsenic was achieved through vermicomposting. The earthworm population growth (2.5-3 folds) was also highly satisfactory in the biochar-based feedstocks. The results clearly imply that Eisenia fetida could compensate the arsenic-induced stress to microbial population and greatly augmented microbial biomass, respiration and enzyme activity by 3-12 folds. Moreover, biochar-induced alkalinity was significantly neutralized in the vermibeds, which remarkably balanced the TOC level and nutrient (N, P, and K) availability particularly in EBC + CD vermibeds. Overall, the nutrient recovery potential and arsenic removal efficiency of vermitechnology was clearly exhibited in NBC/EBC + CD (12.5:87.5) feedstocks. Hence, it is abundantly clear that vermitechnology can be a suitable option for eco-friendly recycling of pollutant-saturated sorbing agents, like biochars.

Epigenetic regulations enhance adaptability and valorization efficiency in Eisenia fetida and Eudrilus eugeniae during vermicomposting of textile sludge: Insights on repair mechanisms of metal-induced genetic damage and oxidative stress.

Genotoxicity-based assessments of vermitechnology for textile-sludge valorization have rarely been attempted. Therefore, waste sanitization and epigenetic stress-regulation efficiency of Eisenia fetida and Eudrilus eugeniae were evaluated in silk (DSPS) and cotton (CPWS) processing sludge-based vermibeds. Vermicomposting resulted in greater C, N, and P recovery than composting. Earthworm population reduced by 6-50% in DSPS/CPWS, while it significantly increased in cow dung (CD) mixed DSPS/CPWS. The Cr, Cd, Pb, and Zn accumulation efficiency of earthworms was higher in DSPS-based feedstocks than CPWS. However, metal-rich sludge elevated oxidative stress, causing greater inhibition of cell viability and DNA damage in Eudrilus than in Eisenia. Although histo-architecture of chloragogenous tissues was perturbed, earthworms combatted metal-induced lipid peroxidation via the activation of catalase, superoxide-dismutase, and reduced-glutathione. Correlation statistics revealed that genetic integrity in earthworms was restored through DNA-methyltransferase activity, especially in DSPS/CPWS + CD vermibeds. Overall, Eisenia was a healthier choice than Eudrilus for sustainable valorization of textile-sludge.

GHRH secretion from a pancreatic neuroendocrine tumor causing gigantism in a patient with MEN1.

SUMMARY: A male patient with a germline mutation in MEN1 presented at the age of 18 with classical features of gigantism. Previously, he had undergone resection of an insulin-secreting pancreatic neuroendocrine tumour (pNET) at the age of 10 years and had subtotal parathyroidectomy due to primary hyperparathyroidism at the age of 15 years. He was found to have significantly elevated serum IGF-1, GH, GHRH and calcitonin levels. Pituitary MRI showed an overall bulky gland with a 3 mm hypoechoic area. Abdominal MRI showed a 27 mm mass in the head of the pancreas and a 6 mm lesion in the tail. Lanreotide-Autogel 120 mg/month reduced GHRH by 45% and IGF-1 by 20%. Following pancreaticoduodenectomy, four NETs were identified with positive GHRH and calcitonin staining and Ki-67 index of 2% in the largest lesion. The pancreas tail lesion was not removed. Post-operatively, GHRH and calcitonin levels were undetectable, IGF-1 levels normalised and GH suppressed normally on glucose challenge. Post-operative fasting glucose and HbA1c levels have remained normal at the last check-up. While adolescent-onset cases of GHRH-secreting pNETs have been described, to the best of our knowledge, this is the first reported case of ectopic GHRH in a paediatric setting leading to gigantism in a patient with MEN1. Our case highlights the importance of distinguishing between pituitary and ectopic causes of gigantism, especially in the setting of MEN1, where paediatric somatotroph adenomas causing gigantism are extremely rare. LEARNING POINTS: It is important to diagnose gigantism and its underlying cause (pituitary vs ectopic) early in order to prevent further growth and avoid unnecessary pituitary surgery. The most common primary tumour sites in ectopic acromegaly include the lung (53%) and the pancreas (34%) (1): 76% of patients with a pNET secreting GHRH showed a MEN1 mutation (1). Plasma GHRH testing is readily available in international laboratories and can be a useful diagnostic tool in distinguishing between pituitary acromegaly mediated by GH and ectopic acromegaly mediated by GHRH. Positive GHRH immunostaining in the NET tissue confirms the diagnosis. Distinguishing between pituitary (somatotroph) hyperplasia secondary to ectopic GHRH and pituitary adenoma is difficult and requires specialist neuroradiology input and consideration, especially in the MEN1 setting. It is important to note that the vast majority of GHRH-secreting tumours (lung, pancreas, phaeochromocytoma) are expected to be visible on cross-sectional imaging (median diameter 55 mm) (1). Therefore, we suggest that a chest X-ray and an abdominal ultrasound checking the adrenal glands and the pancreas should be included in the routine work-up of newly diagnosed acromegaly patients.

Influence of soil quality factors on capsaicin biosynthesis, pungency, yield, and produce quality of chili: An insight on Csy1, Pun1, and Pun12 signaling responses.

Hotness or pungency is the major trait of genetically diverse and economically valuable chili (Capsicum sp.) cultivars. However, little is known about the influence of soil characteristics on genetic regulation of pungency vis-à-vis capsaicin formation in endemic chilies. Hence, the present work was conducted by growing two endemic chili cultivars in two types (alluvial and lateritic) of soil. Capsaicin content, pungency, and capsaicin synthase activity were significantly greater in chilies grown in alluvial soil than in lateritic soil. Correspondingly, Csy1, the gene that encodes capsaicin synthase, was significantly upregulated in alluvial soil grown plants. Interestingly, upregulation of Pun1, the gene responsible for capsaicin accumulation in fruits, was more in lateritic soil than in alluvial soil; but pungency was inhibited in lateritic soil by the overexpression of Pun12, a recessive allele of Pun1 locus. Statistical analyses revealed that high organic C, microbial activity, and NPK status in alluvial soil were responsible for high pungency, capsaicin synthase activity, capsaicin accumulation, and suppression of Pun12. Fruit yield, dry matter, crude protein, titratable acidity, and soluble solids were also significantly high in chilies grown in alluvial soil. Therefore, we postulate that soil quality attributes play vital roles in genetic regulation of pungency, capsaicin biosynthesis, fruit yield, and produce quality of endemic chili cultivars.

Metal induced non-metallothionein protein in earthworm: A new pathway for cadmium detoxification in chloragogenous tissue.

Earthworms neutralize toxic metals by a small (∼13 kDa) cysteine rich metal binding protein, metallothionein (MT). Although the rate of metal accumulation and MT expression does not correlate well, the reason behind such inconsistency has not yet been deciphered. The present investigation clearly demonstrates that expression of some non-MT metal induced proteins is responsible for such incongruity. Applying selective protein isolation techniques in fluorescence tagged cadmium exposed (135 mg/kg) earthworms we were able to purify a 150 kDa metal induced protein (MIP) among others. After 60 days of exposure cadmium accumulation in earthworm intestines was significant. Immunofluorescence staining followed by confocal microscopy exhibited that MIP accumulates ingested cadmium in the intestinal region and eventually deposits the metal in the chloragogenous tissue. We determined the N-terminal sequence of 15 amino acid residues and after bioinformatics analysis, it was concluded that MIP is most probably a glutamic acid rich, novel cadmium binding protein. To further validate the binding mechanism, we conducted paper chromatography and continuous variation experiments which evidenced that cadmium readily binds to glutamic acid. The present finding is the first in-vivo evidence of a non-metallothionein cadmium binding protein induced in the intestines of earthworm exposed to a cadmium rich environment.

Primacy of ecological engineering tools for combating eutrophication: An ecohydrological assessment pathway.

Eutrophication of freshwater bodies causes loss of earth's biological resources and aggravates climate change, thus assuming major environmental concern. Both endogenous and exogenous nutrient enrichment are responsible for eutrophication. Numerous monitoring and management studies conducted worldwide have resulted high-level technological innovations. These studies cumulatively uphold the significance of ecohydrological and ecological engineering approaches. However, holistic and insightful reviews with feasible recommendations of such huge academic outputs are rather scanty. Therefore, our main objective was to introduce a new perspective of eutrophication as an ecohydrological component; to discover all possibilities of monitoring and restoration of eutrophic water bodies. Furthermore, the present study critically analyzes various methods of treatment of eutrophication (physical, biological, chemical, and eco-engineering). Comprehensive volume of literature has been surveyed using search engines like Scopus, Google Scholar, PubMed, ScienceDirect etc. Meaningful keywords were used to obtain reliable information on methods of ecohydrological assessment in relation to eutrophication of freshwater bodies. According to our survey, ecohydrological research is diversified into conceptual knowledge (37.2%), assessment (32.6%), climate change (9.3%), algae/cyanotoxins (7%), engineering and restoration (7%), modelling (4.6%) and biodiversity (2.3%), in the instant decade (2010-2020). We have identified a clear trend of transition of restoration methods from traditional towards modern techniques over time. Moreover, this review recognizes a pool of biophysicochemical and ecological engineering techniques, which are very effective in regard to time, cost, and labor and have immense scopes of modification for improved results. This work focuses on the importance of ecohydrology and eco-engineering tools for restoration of eutrophic water bodies for the first time. We have highlighted how these approaches have emerged as one of the best suitable and sustainable water resource conservation routes in the present era.

Vermiremediation of cotton textile sludge by Eudrilus eugeniae: Insight into metal budgeting, chromium speciation, and humic substance interactions.

Information on prospective metal remediation by Eudrilus eugeniae during vermicomposting of cotton textile sludge (CTS) is rather scarce. This investigation, therefore, evaluates the sanitization efficiency of this species in CTS and CTS + cow-dung (CD) based feedstocks against aerobic composting. Accordingly, reduction in Pb, Cd, Cr, and Zn concentrations was between 50 and 70% under vermicomposting. Budget equations substantiated that humic compound mediated chelation was the dominant route of metal removal, against nominal bioaccumulation by earthworms. Correlation statistics revealed that formation of humic compounds (humic acid, fulvic acid, and humin) greatly influenced the transition of toxic Cr6+ to benign Cr3+ during vermicomposting. Moreover, increase in total N content and P availability was significantly greater under vermicomposting than composting. Thus, E. eugeniae efficiently stabilized the feedstocks by reducing pH, Ca, S, and organic C and CTS + CD(2:1) was the most favorable feedstock for E. eugeniae vermicomposting in respect of metal detoxification and nutrient stabilization.

Secondary metabolites and anti-microbial/anti-oxidant profiles in Ocimum spp.: Role of soil physico-chemical characteristics as eliciting factors.

Ocimum has long been used as a medicinal plant, although little information is available about its bioactive ingredients, and the influence of soil properties on modulation of secondary metabolites in Ocimum has yet to be ascertained. In this study, we present a thorough survey of all potential metabolic compounds in O. sanctum and O. basilicum. In both species, certain compounds (e.g., quercetin, kaempferol, catechin, and S-adenosyl homocysteine) were detected coincidently. In the case of O. basilicum, other vital phenolic acids (e.g., ursolic, vanilic, coumaric, and syringic acids) were identified. The aqueous extracts (AEs) of Ocimum recorded decrease of 6-94% in the proliferation of pathogenic bacteria (e.g., Listeria monocytogenes, Staphylococcus sp., Salmonella sp., and Bacillus sp.). The AEs also showed effective antioxidant activity by reducing free radicals by a factor of 1.04-1.13. Root-zone soil samples of both Ocimum spp. were collected from strategic locations with varying levels of key soil attributes (e.g., soil organic carbon (SOC), microbial biomass carbon (MBC), urease, and phosphatase). At high levels of SOC, MBC, and soil enzymes, the bioactivity of Ocimum spp. was observed to be promoted, especially with respect to secondary metabolite expression, anti-pathogenic activity, and anti-oxidant properties. As such, the findings of strong correlations between secondary metabolite concentrations and bioactivity attributes in Ocimum suggest the potent role of soil quality in eliciting the production of secondary metabolite in association with bioactivity in Ocimum spp.

Detoxification and eco-friendly recycling of brick kiln coal ash using Eisenia fetida: A clean approach through vermitechnology.

Brick kiln coal ashes (BKCAs) are one of the major toxic byproducts of the rapidly growing construction industry in developing countries. However, eco-friendly recycling avenues for BKCAs are yet to be explored. The major objectives of the present research were to evaluate the viability of vermitechnology in transforming BKCAs into valuable products, and to examine the metal detoxification potential of Eisenia fetida BKCA-based feedstocks. BKCAs were mixed in large scale with cow dung (CD) in 1:1 and 2:1 ratios, for vermicomposting and aerobic composting; performance was assessed in comparison with CD. Vermiconverted-BKCA was then used as organic fertilizer for rice grown in poorly fertile soil. Acidic nature of BKCA feedstocks was neutralized by 30-86% in the vermireactors. Total N and available P concentrations significantly increased in the vermireactors supplemented with considerable mineralization of total organic C. Exorbitantly high K and S contents were pacified to a normal range after vermicomposting. Greater improvement in microbial biomass, respiration, fungal and bacterial growth was observed under vermicomposting against aerobic composting. Consequently, urease and phosphatase activity increased by 1-4 folds in the BKCA based vermibeds. Bioavailability of toxic metals reduced by 41-74% in the vermicomposted BKCAs. High metal accumulation by the earthworms resulted in substantial reduction of pollution load in the finished product. The field experiment demonstrated that vermiconverted-BKCA could be utilized as potential organic fertilizer for rice production, soil fertility rejuvenation, and metal detoxification. Overall, the study reveals that E. fetida could be used as an efficient contender for sanitization of toxic BKCAs.